Fig. 4

Measurement of von Willebrand factor (vWF) multimers by immunoblotting in whole blood subjected to high and low flow conditions. Representative immunoblot image from two gels run at high flow and low flow (a) with loss of high-molecular-weight (HMW) vWF bands (analysed by densitometry). Data normalized to total vWF for HMW multimers (b) and LMW multimers (c). Data are presented as mean ± SEM. **p < 0.01 from baseline to 6 h; #p < 0.05 high vs low flow. n = 5 high flow, n = 4 low flow