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Intensive Care Medicine Experimental
Fig. 2 | Intensive Care Medicine Experimental

Fig. 2

From: Intracellular calcium signaling and phospho-antigen measurements reveal functional proximal TCR activation in lymphocytes from septic shock patients

Fig. 2

PI3/Akt/mTOR pathway activation in CD4+ T cells from septic patients and controls upon TCR ligation. PBMCs were isolated from septic patients and controls, stimulated for 7 min with anti-CD2-CD3-CD28 Ab-coated beads (bead/cell ratio = 3/1) and stained with anti-CD4 and anti-CD3z, anti-pS6, anti-pAkt, anti-pERk, and anti-pAMPK antibodies protein phosphorylation was assessed by flow cytometry. Data represent the percentage of positive cells based on FMO staining and as individual values and means +/− SD in 9 septic patients and 9 healthy volunteers (controls) before (baseline, BL) and after TCR stimulation (TCR stim). *p < 0.05 (Wilcoxon paired test, baseline vs TCR stim), #p < 0.05 (Mann-Whitney, healthy volunteers vs patients after stimulation)

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