Skip to main content
Intensive Care Medicine Experimental
Fig. 3 | Intensive Care Medicine Experimental

Fig. 3

From: Vendor effects on murine gut microbiota and its influence on lipopolysaccharide-induced lung inflammation and Gram-negative pneumonia

Fig. 3

Experimental model of LPS inoculation and following cytokine and cell response. a Experimental design. Fecal samples were obtained from mice from 3 different vendors (Janvier, Envigo, and Charles River) prior to intranasal inoculation with 1 μg lipopolysaccharide (LPS) from K. pneumoniae. Mice were sacrificed at 6 and 24 h post-inoculation. Bronchoalveolar lavage fluid (BALF) was analyzed using flow cytometry. The graphs show the percentages of CD45-positive cells that are alveolar macrophages (a) or neutrophils (b). Tumor necrosis factor alpha (TNFα) (d, e) and interleukin 6 (IL-6) (f, g) in lung homogenate (e, g) and bronchoalveolar lavage fluid (BALF) (d, f) at 6 h and 24 h post-inoculation. The abbreviations used to indicate vendors are as follows: Janvier (Jan), Envigo (Env), and Charles River (CR). Results are shown as mean ± s.e.m. (n = 5–8), and ns denotes not significant. p < 0.05 (*) and p < 0.01 (**)

Back to article page