Fig. 1
From: Triiodothyronine prevents tissue hypoxia in experimental sepsis: potential therapeutic implications
Representative microscopy images after pimonidazole (PMZ) staining from heart and liver tissues of naive (a and d), placebo (b and e) and T3-treated (c and f) animals. Detection of hypoxia (PMZ positive) was based on immunoperoxidase staining (brown color). Analysis of the pictures for detection of the PMZ-positive signal and area was performed in ImageJ using the "Threshold Color" function. Selection of threshold values was based on stained sections from naïve animals. "PMZ-positive" % area of the heart and liver tissue was identified as the percentage of pixels with color above the set threshold to total pixels in each picture. Background areas with no tissue were removed from the calculation. g Lactate levels in serum were 5.2 ± 0.28 mM in placebo and 4.2 ± 0.35 in T3-treated group 18 h after surgery, p < 0.05. Baseline lactate levels in naive animals were 4.1 ± 0.3 mM, p < 0.05 vs placebo only. h Quantification of PMZ-positive staining in left ventricular tissue was 4% ± 0.5 in placebo and 1.5% ± 0.5 in T3-treated hearts 18 h after surgery, p = 0.028. i PMZ-positive staining in liver was 3.8% ± 1.4 in placebo and 0.3% ± 0.1 in T3-treated hearts, p = 0.026. Results are presented as mean ± SEM. *p < 0.05 vs placebo