Fig. 1
From: Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model
Effects of cecal ligation and puncture protocol and mechanical ventilation on arterial blood pressure, cytokines, and mRNA levels. Rats underwent cecal ligation and puncture (CLP) (n = 12) or sham operation (n = 12) at time 0. Animals were resuscitated with normal saline (10 ml.kg−1.h) intravenously. Half of the CLP and sham rats were intubated and mechanically ventilated and assigned to one of four groups (n = 6 per group): (1) a spontaneously breathing control group (control), (2) a spontaneously breathing septic group (sepsis), (3) a mechanically ventilated control group (MV-control), and (4) a mechanically ventilated septic group (MV-sepsis). a Shows the mean arterial blood pressure (MAP mmHg) throughout the study in control groups (open circles) and sepsis groups (dark circles). Data are means ± SEM from 6 animals of each group. *p < 0.05 sepsis versus control; ‡ < 0.05 MV-sepsis versus control; †p < 0.05 sepsis versus MV-control; §p < 0.05 MV-sepsis versus MV-control. Panel B shows diaphragm interleukin 1 beta (IL-1β) mRNA expression (b). Transcript levels of each group were standardized with mRNA level of 18 S. Data are means ± SEM from 4 animals per group. *p < 0.05 sepsis versus control; ‡ < 0.05 MV-sepsis versus control; †p < 0.05 sepsis versus MV-control; §p < 0.05 MV-sepsis versus MV-control for 4 animals per group