Fig. 3

Cytochrome c oxidase activity and subunit 1 content. The maximal activity of cytochrome c oxidase (a) and representative western blot of cytochrome c oxidase subunit 1 (b). Cytochrome c oxidase was assessed by the addition of 2 mM ascorbate plus 500 μM N,N,N’N’-tetramethyl-phenylenediamine (TMPD) in the presence of 3 μM myxothiazol. Values are means ± SEM from 6 animals in control, sepsis, MV-sepsis, and MV-control. *p < 0.05 sepsis versus control; ^†p < 0.05 sepsis versus MV-control; ^£p < 0.05 sepsis versus MV-sepsis. Western blot analysis of diaphragm mitochondrial cytochrome c oxidase subunit 1 (Panel B). Data are means ± SEM from 4 animals per group. *p < 0.05 sepsis versus control; ^†p < 0.05 sepsis versus MV-control; ^£p < 0.05 sepsis versus MV-sepsis; ^¡p < 0.05 control versus MV-control; ^§p < 0.05 MV-sepsis versus MV-control