Fig. 4

Diaphragm mitochondrial H₂O₂ production and MDA content. a Shows H₂O₂ measured in the presence of 0.3% free fatty acid–BSA and 5 mM succinate without rotenone (succinate), then ADP (succinate-state 3) added to increase mitochondrial activity ATP generation and inhibit H₂O₂ generation, and then rotenone (rotenone) added to measure radical production at the level of complex 3, as described in “Materials and methods” section. Data are means ± SEM from 4 animals per group. *p < 0.05 sepsis versus control; ^†p < 0.05 sepsis versus MV-control; ^£p < 0.05 sepsis versus MV-sepsis. b Shows MDA (malondialdehyde) content in the diaphragm of control, sepsis, MV-sepsis, and MV-control. Six hours after CLP, diaphragms were removed to determine thiobarbituric acid-reactive species content. Data are means ± SEM from 6 animals per group. *p < 0.05 sepsis versus control; ^†p < 0.05 sepsis versus MV-control; ^‡p < 0.05 MV-sepsis versus control; ^§p < 0.05 MV-sepsis versus MV-control; ^£p < 0.05 sepsis versus MV-sepsis.