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Table 2 Mitochondrial oxygen consumption and ATP synthesis

From: Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model

  Control Sepsis MV-control MV-sepsis
State 3 respiration (natm/min/mg) 42±3 25±3*†₤ 40±2 34±4
State 4 respiration (natm/min/mg) 12.8±3.9 6.2±1.1 11.1±1.5 8.8±2.4
RCR 4.18±0.81 4.32±0.53 3.83±0.55 4.04±0.72
ATP production 119±21 53±15*†₤ 130±23 95±10
ATP/O 2.75±0.50 2.20±0.60 3.23±0.62 2.86±0.41
  1. Measures were performed on diaphragm mitochondria from rats of the four following groups: spontaneously breathing controls (Control), spontaneously breathing sepsis group (Sepsis), mechanically ventilated control group (MV-control) and a mechanically ventilated sepsis group (MV-sepsis). Mitochondria were energized with succinate as respiratory substrate in the presence of rotenone State 3 phosphorylating respiration as well as ATP production were measured at 100 µM of exogenous ADP. State 4 respiration was obtained by inhibiting the ATP synthase with 3 µg/ml of oligomycin. Data are means ± SEM from 6 animals per group. *p < 0.05 sepsis versus Control; p < 0.05 sepsis versus MV-control; £p < 0.05 sepsis versus MV-sepsis