The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats

van Leeuwen, Anoek L. I.; Dekker, Nicole A. M.; Van Slyke, Paul; de Groot, Esther; Vervloet, Marc G.; Roelofs, Joris J. T. H.; van Meurs, Matijs; van den Brom, Charissa E.

doi:10.1186/s40635-021-00389-5

Intensive Care Medicine Experimental

Table 2 Cremaster perfusion and renal perfusion data

From: The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats

Parameter	Group	Time point
		Baseline	0.5 h HS	1 h HS	R + 0.5 h	R + 1 h	R + 2 h	R + 3 h	R + 4 h
Cremaster perfusion
Continuously perfused vessels (vessels/recording)	HS	12 ± 2	7 ± 3*	5 ± 2*	9 ± 2*	8 ± 2*	8 ± 3*	8 ± 3*	7 ± 2*
Continuously perfused vessels (vessels/recording)	HS + VT	13 ± 1	7 ± 3*	4 ± 2*	11 ± 2*	10 ± 2*	10 ± 3*	11 ± 3*^	10 ± 3*
Intermittently perfused vessels (vessels/recording)	HS	0.6 ± 0.3	1.0 ± 0.5	1.3 ± 0.6*	0.9 ± 0.6	0.9 ± 0.5	0.9 ± 0.4	0.9 ± 0.6	0.8 ± 0.4
Intermittently perfused vessels (vessels/recording)	HS + VT	0.5 ± 0.4	1.0 ± 0.6	0.9 ± 0.4	0.8 ± 0.5	1.0 ± 0.8	0.8 ± 0.7	0.8 ± 0.9	0.8 ± 1.0
Non-perfused vessels (vessels/recording)	HS	4 ± 1	8 ± 3*	10 ± 1*	7 ± 3*	7 ± 3*	8 ± 3*	8 ± 2*	8 ± 2*
Non-perfused vessels (vessels/recording)	HS + VT	4 ± 1	9 ± 3*	11 ± 2*	6 ± 2*	7 ± 2*	7 ± 2*	6 ± 2*	6 ± 2*
Renal perfusion
Microvascular blood volume A (a.u.)	HS	206 ± 149	178 ± 258	146 ± 149	254 ± 196	253 ± 211	215 ± 142	243 ± 233	246 ± 149
Microvascular blood volume A (a.u.)	HS + VT	201 ± 101	178 ± 199	217 ± 234	340 ± 318	380 ± 408	309 ± 302	253 ± 190	217 ± 149
Microvascular filling velocity B (s⁻¹)	HS	1.3 ± 0.7	0.5 ± 0.3*	0.4 ± 0.2*	0.7 ± 0.2*	0.7 ± 0.8*	0.6 ± 0.2*	0.6 ± 0.2*	0.5 ± 0.2*
Microvascular filling velocity B (s⁻¹)	HS + VT	1.2 ± 0.2	0.5 ± 0.1*	0.4 ± 0.1*	0.7 ± 0.2*	0.7 ± 0.2*	0.5 ± 0.2*	0.5 ± 0.2*	0.7 ± 0.3*
Estimate of perfusion	HS	241 ± 139	59 ± 52*	51 ± 41*	177 ± 131	158 ± 107	119 ± 63	113 ± 59	120 ± 58
Estimate of perfusion	HS + VT	234 ± 122	100 ± 122*	83 ± 89*	242 ± 222	238 ± 222	148 ± 115	118 ± 81	146 ± 105

Data presented as mean ± SD. * P < 0.05 compared to baseline value of the corresponding group. ^ P < 0.05 vasculotide effect, compared to the same time point of the untreated HS group. HS; hemorrhagic shock group, HS + VT; hemorrhagic shock group treated with vasculotide. Time points represent 0.5 h following start of blood withdrawal (0.5 h HS), 1 h following start of blood withdrawal (1 h HS) and 0.5 h (R + 0.5 h), 1 h (R + 1 h), 2 h (R + 2 h), 3 h (R + 3 h) and 4 h (R + 4 h) following start of fluid resuscitation

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