Fig. 2
Ex vivo assessment of femoral artery reactivity using wire myography in HIF-1α Tie2 Cre mice. Femoral artery reactivity was assessed using wire myography in HIF-1α Tie2 Cre (green, n = 5) and HIF-1α flox/flox littermates (grey, n = 5). Data presented as Mean ± SEM. For concentration response curves, analysis was by two-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001). The NOS-dependent/independent components expressed as area under the curve were analysed by One-way ANOVA with Tukey test (*p < 0.05 vs. NOS-dependent, #p < 0.05 vs. NOS-independent, †p < 0.05 vs. total effect). Data for the KCl, Phe and SNP area under the curve were analysed by the Student’s t test for unpaired data (*p < 0.05). a Degree of relaxation was expressed as a percentage of the contraction induced by phenylephrine. [ACh]: molar concentration of acetylcholine. b The contribution of NO-independent mechanisms was calculated by the AUC for ACh + LNAME (10−5 M), *p < 0.05 for NO (Nitric Oxide) dependent and # p < 0.05 for NO-independent portions of ACh-induced relaxation. c Degree of relaxation was expressed as a percentage of the contraction induced by phenylephrine. [SNP]: molar concentration of sodium nitroprusside and d degree of vasoconstriction developed in response to increasing molar concentrations of phenylephrine ([Phe] 10−5 M)