Fig. 3

Effects of HIF-1α pulmonary endothelial knockout on constitutive cardiovascular function. Circadian variations in a systolic, b diastolic blood pressure, c heart rate, d subcutaneous temperature, e VO₂ and f VCO₂ of HIF-1α L1 Cre (red, n = 11) and littermate HIF-1α flox/flox (grey, n = 9) mice were recorded by radio-telemetry. Black box represents nocturnal phase. Data are presented as a mean ± SEM for each 30 min period, p value for area under the curve followed by unpaired t test are shown. Femoral artery reactivity was assessed using wire myography in HIF-1α L1Cre (red, n = 5) and HIF-1α flox/flox littermates (grey, n = 5). g Degree of relaxation was expressed as a percentage of the contraction induced by phenylephrine. [ACh]: molar concentration of acetylcholine. h Degree of relaxation was expressed as a percentage of the contraction induced by phenylephrine. [SNP]: molar concentration of sodium nitroprusside and i degree of vasoconstriction developed in response to increasing molar concentrations of phenylephrine ([Phe] 10⁻⁵ M). Data presented as Mean ± SEM for concentration response, analysis by two-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001)