Fig. 4

Effects of HIF-1α pulmonary endothelial knockout on response to acute hypoxia and inflammatory stress. Impact of acute hypoxia with inspired oxygen concentration of 10% on a systolic (p = 0.16), b diastolic blood pressure (p < 0.001), c heart rate (p = 0.473), d subcutaneous temperature (p < 0.001), e oxygen consumption (p < 0.01) and f carbon dioxide synthesis (p = 0.50) of HIF-1α L1 Cre (red, n = 5) and littermate HIF-1α flox/flox (grey, n = 6) mice recorded by radio-telemetry. p value reflects one-phase association non-linear regression from nadir value after the onset of hypoxia. Impact of a 10 mg/kg bolus of LPS on g systolic (p = 0.01) and h diastolic (p = 0.15) (h) blood pressures and heart rate (p = 0.25) (i) in HIF-1α L1 Cre (red, n = 3) and littermate HIF-1α flox/flox (grey, n = 3) mice using continuous radio-telemetry is reported. Data are presented as a mean ± SEM) for each 30 min period. Analysis of recovery trajectory after initial hypoxia exposure by one-phase association fitting, haemodynamic data following LPS bolus are presented as a mean ± SEM for each 30-min period, p value represents analysis of area under the curve for each animal followed by unpaired t test