Fig. 1

Schematic overview and perioperative hemodynamics. Rats were randomized to undergo cardiopulmonary bypass with PBS (CPB control, n = 15) or cardiopulmonary bypass with aprotinin treatment (CPB + AP, n = 15). AP or PBS were administered after baseline measurements were performed and a secondary dose was given 60 min after onset of CPB. Microcirculatory perfusion measurements in the cremaster muscle (yellow circles) were performed directly after the surgical preparation before onset of CPB (baseline), 10 min after initiation of CPB (10 min CPB), 60 min after initiation of CPB (60 min CPB), 10 min after weaning from CPB (10 min post-CPB) and 60 min after weaning from CPB (60 min post-CPB). Renal perfusion measurements (red circles) were performed before onset of CPB (baseline), 10 min after weaning from CPB (10 min post-CPB) and 60 min after weaning from CPB (60 min post-CPB). Plasma was collected at baseline, 60 after initiation of CPB (60 min CPB) and 60 min after weaning from CPB (60 min post-CPB). Rats were culled 60 min after weaning from CPB and urine samples and kidneys were stored for further analyses (a). Mean arterial pressure (b) and hematocrit levels (c) in rats during and following cardiopulmonary bypass (CPB; white boxes; n = 15) or CPB with aprotinin treatment (CPB + AP; red boxes; n = 15). Boxes and whiskers represent median, interquartile and full range, *p < 0.05 CPB vs. CPB baseline, #p < 0.05 CPB vs. CPB + AP