Fig. 3
From: Pulmonary and intestinal microbiota dynamics during Gram-negative pneumonia-derived sepsis
source tracking (FEAST) analysis on the lung microbiota showing which percentage of the lung microbiota can be traced back to the tongue, feces and unknown source at 0, 12 and 30Â h post-inoculation, in a dataset without K. pneumoniae
Alpha and beta diversity of the lung, tongue and fecal microbiotas. Mice received an intranasal inoculation with 104 colony forming units (CFU) of K. pneumoniae. Mice were killed at 0, 12, and 30 h post-inoculation (n = 12), lung tongue and feces were extracted for microbiota analysis. a Alpha diversity; Shannon of lung tongue and fecal samples at 0, 12 and 30 h post-inoculation. b Beta diversity; PCoA Bray analysis of lung tongue and feces over time. c Genus distribution for lung tongue and fecal microbiota at 0, 12, 30 h post-inoculation, where the top 10 genera in the lung are colored, other genera are grey. d Fast expectation–maximization for microbial