Fig. 4.

GATA3 regulates Tie2 expression and vascular permeability in vivo. Graph showing (A) GATA3 mRNA and (B) Tie2 mRNA expression in ECs isolated from the lungs of endothelial GATA3 knock-out mice and controls (***p < 0.001, n = 7 per group). C Representative immunoblot of whole-lung lysates for Tie2 expression in endothelial GATA3 knock-out mice and control mice (D) densitometric quantification of blots (*p < 0.05, n = 5 per group). E Periodic acid-Schiff (PAS) staining of paraffin-embedded lung tissue in clonidine-induced hypotension mice and saline-treated controls. All images show bronchus (b), their corresponding arteriola (a) and the peribronchial cuffing (a surrogate of lung edema) The schematic illustration below highlights this peribronchial cuff area (in red). F) Semi-quantification of peribronchial cuffing was performed by surveying whole lung sections. (*p < 0.05, n = 4 per group). G Lung wet-to-dry weight ratio of saline-treated controls and clonidine-induced hypotension group (*p < 0.05, n = 8 per group)