Fig. 5.

Pulmonary GATA3 overexpression attenuates loss of Tie2, thereby suppressing vascular leakage in vivo. A GATA3 mRNA and (B) Tie2 mRNA expression in the lungs of GATA3 overexpressing (jetPEI GATA3) and control mice with (clonidine) and without (saline) the induction of hypotension (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant, n = 6 per group). C Representative immunoblot of whole-lung lysates for Tie2 protein expression in GATA3 overexpressing (jetPEI GATA3) and control mice. D Densitometric quantification of blots (*p < 0.05, n = 4 per group). E Periodic acid-Schiff (PAS) staining of paraffin-embedded lung tissue in GATA3 overexpressed and control mice. All images show bronchus (b), their corresponding arteriola (a) and the peribronchial cuffing (a surrogate of lung edema) The schematic illustration below highlights this peribronchial cuff area (in red). F Semi-quantification of peribronchial cuffing was performed by surveying whole lung sections. (*p < 0.05, **p < 0.01, ns = not significant. n = 4 per group)