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Fig. 5 | Intensive Care Medicine Experimental

Fig. 5

From: Effects of therapeutic plasma exchange on the endothelial glycocalyx in septic shock

Fig. 5

Ex vivo stimulation of 3D microvessels with septic serum before and after therapeutic plasma exchange (TPE). Exemplary 3D reconstruction of the heparan sulfate (HS) layer images of naive endothelial cells in a microfluidic chip (HS in red, DAPI nuclei staining in blue) after perfusion with serum and incubation overnight of a representative septic patient both before and after TPE compared to healthy control serum (pooled out of four control patients) (A) and quantification of the HS positive surface area as box and whisker plots (B)

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