Fig. 4

Systemic inflammation in donor animals. Changes in plasma a) total white blood cells (WBC, 10⁹/L); b) big endothelin-1 (BET-1, pg/mL); c) interleukin 1β (pg/mL); d interleukin-6 (pg/mL); e interleukin-8 (pg/mL); f interleukin-10 (pg/mL); and g tumour necrosis factor alpha (TNFα, ng/mL) following confirmation of donor BSD (or sham). Data are mean ± SEM, n = 4/group. Top F and p values on each graph represent effect of time, and bottom F and p values represent differences between BSD vs. sham over time. B Baseline, ST stabilisation 1 h following completion of instrumentation procedures in donor, 0H—confirmation of BSD. Dotted lines on c and g represent the lowest detectable limit of the respective cytokine ELISA