Fig. 9

Loss of TNFα shedding in iRHOM2 deficiency and cardiac inflammation and contractility after polymicrobial sepsis. A Cardiac TNFα gene transcription in naïve and septic RHOM2 deficient mice (n = 5/sex). B TNFR1/R2 ratio of mRNA expression in female and male iRHOM2-deficient mice before and after sepsis (n = 5/sex). C mRNA expression of sarco(endo)plasmic reticulum Ca2 + ATPase 2 (SERCA2) female and male iRHOM2-deficient mice before and after sepsis (n = 5/sex). D Immunoblots showing comparison between wild-type and knockout mice for phosphotroponin I protein expression 18 h after. Β-actin corrected values are compared in summary graph for 8/mice each sex. Individual p values on each panel refer to post hoc testing after ANOVA testing for sex x gene interaction. E Immunoblot for phospholamban protein expression in left ventricle 18 h after sepsis in wild-type male and female mice (original uncropped version in Additional file 1). Β-actin corrected values are compared in summary graph for 8/mice each sex. Individual p values on each panel refer to post hoc testing after ANOVA testing for sex x gene interaction. F A higher proportion of CD4 + cells infiltrating the left ventricle in male iRHOM2^−/− mice (31.9 ± 5.1% CD45 + cells; n = 5), compared to 13.4 ± 3.4% (n = 5) in female iRHOM2^−/− mice (p = 0.02). Individual p values on each panel refer to post hoc testing after ANOVA testing for sex x time interaction