Fig. 2
From: Effect of noradrenaline on propofol-induced mitochondrial dysfunction in human skeletal muscle cells
A and B Analysis of mitochondrial mass by confocal imaging of exposed myoblasts. A Representative confocal images of each channel after dual staining with Mitotracker Green™ FM (accumulating in mitochondria) and CellMask™ Deep Red (binding into the cell membrane). Additionally, all cells were stained with nuclear blue-fluorescent probe NucBlue. Experiments were performed at least at 50 cells per each condition from 3 independent measurements (= cells established from 3 individual subjects). B Mitochondrial mass calculated as a fraction (%) of a cell surface area in 2D cross-sectional images. Mitochondrial mass (mitochondrial footprint) was analysed as a sum of positive pixels (binary image) per cell representing mitochondrial area using ImageJ™ tool “MINA”. C Activity of CS enzyme measured spectrophotometrically. D Total protein content from frozen cell pellets was determined using Bradford assay as described elsewhere [53]. For both CS activity and protein content measurement, experiments were performed in n = 7 replicates in tri- or tetraplicates. Error bars indicate standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control group. CS citrate synthase, MTG MitoTracker Green™ FM, NA noradrenaline