Articles

1071. Impact of volume resuscitation on heart rate variability in a model of hemorrhagic shock in pigs

Authors: E Salomão Jr, DA Otsuki, AL Corrêa, DT Fantoni and JOC Auler Jr

1069. Pharmacological preconditioning with vitamin c attenuates intestinal injury via the induction of heme oxygenase-1 after hemorrhagic shock in rats

Authors: B Zhao, J Fei, Y Chen, X-Q Song, L Ma, L Wang, E-Z Chen and E-Q Mao

0730. Plasma endocan levels are associated with endothelial dysfunction during experimental human endotoxemia

Authors: LT van Eijk, LAE Cox, BPC Ramakers, MJ Dorresteijn, J Gerretsen, M Kox and P Pickkers

0714. Interaction between adipokines and the metabolic stress response: angpt l2 cxcl5 and visfatin in patients undergoing cardiac surgery

Authors: T Meyer, E Nitschmann, U Schurr, A Boltres, C Bläsi, L Wykes, H Pargger and A Kopp Lugli

0371. Temporal changes in systemic and renal inflammation and histology in a 72-hour rat model of faecal peritonitis

Authors: N Arulkumaran, M Sixma, E Ceravola, R Unwin, FW Tam and M Singer

0353. Do the noble gases helium and argon exert neuroprotective effects in a rodent cardiac arrest model?

Authors: P Zuercher, D Springe, A Putzu, D Grandgirard, S Leib, SM Jakob, J Takala and M Haenggi

Brain function in iNOS knock out or iNOS inhibited (l-NIL) mice under endotoxic shock

Microcirculatory dysfunction due to excessive nitric oxide production by the inducible nitric oxide synthase (iNOS) is often seen as a motor of sepsis-related organ dysfunction. Thus, blocking iNOS may improve...

Authors: Hanna Schweighöfer, Christoph Rummel, Konstantin Mayer and Bernhard Rosengarten

Can computer simulators accurately represent the pathophysiology of individual COPD patients?

Computer simulation models could play a key role in developing novel therapeutic strategies for patients with chronic obstructive pulmonary disease (COPD) if they can be shown to accurately represent the patho...

Authors: Wenfei Wang, Anup Das, Tayyba Ali, Oanna Cole, Marc Chikhani, Mainul Haque, Jonathan G Hardman and Declan G Bates

Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)

Neutrophil extracellular traps (NETs) are a central player in the host response to bacteria: neutrophils release extracellular DNA (nucleosomes) and neutrophil elastase to entrap and kill bacteria. We studied ...

Authors: Hanna K de Jong, Gavin CKW Koh, Ahmed Achouiti, Anne J van der Meer, Ingrid Bulder, Femke Stephan, Joris JTH Roelofs, Nick PJ Day, Sharon J Peacock, Sacha Zeerleder and W Joost Wiersinga

Early and dynamic changes in gene expression in septic shock patients: a genome-wide approach

As early and appropriate care of severe septic patients is associated with better outcome, understanding of the very first events in the disease process is needed. Pan-genomic analyses offer an interesting opp...

Authors: Marie-Angélique Cazalis, Alain Lepape, Fabienne Venet, Florence Frager, Bruno Mougin, Hélène Vallin, Malick Paye, Alexandre Pachot and Guillaume Monneret

The receptor for advanced glycation end products in ventilator-induced lung injury

Mechanical ventilation (MV) can cause ventilator-induced lung injury (VILI). The innate immune response mediates this iatrogenic inflammatory condition. The receptor for advanced glycation end products (RAGE) ...

Authors: Maria T Kuipers, Hamid Aslami, Pieter Roel Tuinman, Anita M Tuip-de Boer, Geartsje Jongsma, Koenraad F van der Sluijs, Goda Choi, Esther K Wolthuis, Joris JTH Roelofs, Paul Bresser, Marcus J Schultz, Tom van der Poll and Catharina W Wieland

A standardized model of brain death, donor treatment, and lung transplantation for studies on organ preservation and reconditioning

We set a model of brain death, donor management, and lung transplantation for studies on lung preservation and reconditioning before transplantation.

Authors: Franco Valenza, Silvia Coppola, Sara Froio, Giulia Maria Ruggeri, Jacopo Fumagalli, Alessandro Maria Villa, Lorenzo Rosso, Paolo Mendogni, Grazia Conte, Caterina Lonati, Andrea Carlin, Patrizia Leonardi, Stefano Gatti, Nino Stocchetti and Luciano Gattinoni

Effects of glycemic control on glucose utilization and mitochondrial respiration during resuscitated murine septic shock

This study aims to test the hypothesis whether lowering glycemia improves mitochondrial function and thereby attenuates apoptotic cell death during resuscitated murine septic shock.

Authors: Josef A Vogt, Ulrich Wachter, Katja Wagner, Enrico Calzia, Michael Gröger, Sandra Weber, Bettina Stahl, Michael Georgieff, Pierre Asfar, Eric Fontaine, Peter Radermacher, Xavier M Leverve and Florian Wagner

Regulation of lung oxidative damage by endogenous superoxide dismutase in sepsis

The purpose of this research is to study the relationship between superoxide dismutase (SOD) and lung redox state in an animal model of sepsis.

Authors: Larissa Constantino, Renata Casagrande Gonçalves, Vinícius Renê Giombelli, Cristiane Damiani Tomasi, Francieli Vuolo, Luiza Wilges Kist, Giovanna Medeiros Tavares de Oliveira, Matheus Augusto de Bittencourt Pasquali, Maurício Reis Bogo, Thais Mauad, Adolfo Horn Jr, Karen V Melo, Christiane Fernandes, José Cláudio Fonseca Moreira, Cristiane Ritter and Felipe Dal-Pizzol

Relevance of induced and accidental hypothermia after trauma-haemorrhage–what do we know from experimental models in pigs?

Recent experimental research has either focused on the role of accidental hypothermia as part of the lethal triad after trauma or tried to elucidate the effects of therapeutically induced hypothermia on the po...

Authors: Frank Hildebrand, Peter Radermacher, Steffen Ruchholtz, Markus Huber-Lang, Andreas Seekamp, Sascha Flohé, Martijn van Griensven, Hagen Andruszkow and Hans-Christoph Pape

‘Chronomics’ in ICU: circadian aspects of immune response and therapeutic perspectives in the critically ill

Complex interrelations exist between the master central clock, located in the suprachiasmatic nuclei of the hypothalamus, and several peripheral clocks, such as those found in different immune cells of the bod...

Authors: Vasilios Papaioannou, Alexandre Mebazaa, Benoît Plaud and Matthieu Legrand

Genetic and pharmacologic inhibition of Tpl2 kinase is protective in a mouse model of ventilator-induced lung injury

Mechanical stress induced by injurious ventilation leads to pro-inflammatory cytokine production and lung injury. The extracellular-signal-regulated-kinase, ERK1/2, participates in the signaling pathways activ...

Authors: Evangelos Kaniaris, Katerina Vaporidi, Eleni Vergadi, Emmanuel E Theodorakis, Eumorfia Kondili, Eleni Lagoudaki, Christos Tsatsanis and Dimitris Georgopoulos

Moderately high frequency ventilation with a conventional ventilator allows reduction of tidal volume without increasing mean airway pressure

The aim of this study was to explore if positive-pressure ventilation delivered by a conventional ICU ventilator at a moderately high frequency (HFPPV) allows a safe reduction of tidal volume (V_T) below 6 mL/kg i...

Authors: Ricardo Luiz Cordioli, Marcelo Park, Eduardo Leite Vieira Costa, Susimeire Gomes, Laurent Brochard, Marcelo Britto Passos Amato and Luciano Cesar Pontes Azevedo

Statins, fibrates, thiazolidinediones and resveratrol as adjunctive therapies in sepsis: could mitochondria be a common target?

Through their pleiotropic actions, statins, fibrates, thiazolidinediones and resveratrol can target multiple mechanisms involved in sepsis. Their actions on mitochondrial function are of interest in a patholog...

Authors: Jerome Morel and Mervyn Singer

Assessment of ventilation inhomogeneity during mechanical ventilation using a rapid-response oxygen sensor-based oxygen washout method

Ventilatory inhomogeneity indexes in critically ill mechanically ventilated patients could be of importance to optimize ventilator settings in order to reduce additional lung injury. The present study compared...

Authors: Ido G Bikker, Wim Holland, Patricia Specht, Can Ince and Diederik Gommers

Reliability of the nitrogen washin-washout technique to assess end-expiratory lung volume at variable PEEP and tidal volumes

End-expiratory lung volume measurement by the nitrogen washin-washout technique (EELV_WI-WO) may help titrating positive end-expiratory pressure (PEEP) during acute respiratory distress syndrome (ARDS). Validation...

Authors: Jean-Christophe Richard, Céline Pouzot, Alfredo Morales Pinzón, Juan Sebastian Torres González, Maciej Orkisz, Bruno Neyran, Marcela Hernández Hoyos, Franck Lavenne and Claude Guerin

The effect of vitamin C on plasma volume in the early stage of sepsis in the rat

Previous experimental studies have shown that vitamin C has several beneficial effects in sepsis and burns, such as decreased tissue oedema, improved endothelial barrier function and decreased transcapillary l...

Authors: Björn P Bark and Per-Olof Grände

Uncontrolled sepsis: a systematic review of translational immunology studies in intensive care medicine

The design of clinical immunology studies in sepsis presents several fundamental challenges to improving the translational understanding of pathologic mechanisms. We undertook a systematic review of bed-to-ben...

Authors: David J Cain, Ana Gutierrez del Arroyo and Gareth L Ackland

Bis maltolato oxovanadium (BMOV) and ischemia/reperfusion-induced acute kidney injury in rats

The aim of the present study was to test the potential protective effects of the organic vanadium salt bis (maltolato) oxovanadium (BMOV; 15 mg/kg) in the context of renal ischemia/reperfusion (30 min of ische...

Authors: Emre Almac, Rick Bezemer, Asli Kandil, Ugur Aksu, Dan MJ Milstein, Jan Bakker, Cihan Demirci-Tansel and Can Ince

Administration of intrapulmonary sodium polyacrylate to induce lung injury for the development of a porcine model of early acute respiratory distress syndrome

The loss of alveolar epithelial and endothelial integrity is a central component in acute respiratory distress syndrome (ARDS); however, experimental models investigating the mechanisms of epithelial injury ar...

Authors: William R Henderson, Julian Barnbrook, Paolo B Dominelli, Donald EG Griesdale, Tara Arndt, Yannick Molgat-Seon, Glen Foster, Gareth L Ackland, James Xu, Najib T Ayas and Andrew W Sheel

Acute organ injury is associated with alterations in the cell-free plasma transcriptome

Despite a genomic revolution in biological sciences, clinical medicine has yet to integrate diagnostics based upon gene expression into practice. While commonly used plasma protein assays rely on organ-specifi...

Authors: John H Boyd, Melissa McConechy and Keith R Walley

Mechanical ventilation with heliox in an animal model of acute respiratory distress syndrome

Heliox has a lower density and higher diffusion capacity compared to oxygen-in-air. We hypothesized that heliox ventilation allows for a reduction in minute volume ventilation and inspiratory pressures needed ...

Authors: Charlotte J Beurskens, Hamid Aslami, Friso M de Beer, Joris JTH Roelofs, Margreeth B Vroom and Nicole P Juffermans

A novel echocardiographic imaging technique, intracatheter echocardiography, to guide veno-venous extracorporeal membrane oxygenation cannulae placement in a validated ovine model

Echocardiography plays a fundamental role in cannulae insertion and positioning for extracorporeal membrane oxygenation (ECMO). Optimal access and return cannulae orientation is required to prevent recirculati...

Authors: David G Platts, Andrew Hilton, Sara Diab, Charles McDonald, Matthew Tunbridge, Saul Chemonges, Kimble R Dunster, Kiran Shekar, Darryl J Burstow and John F Fraser

Unilateral mechanical asymmetry: positional effects on lung volumes and transpulmonary pressure

Ventilated patients with asymmetry of lung or chest wall mechanics may be vulnerable to differing lung stresses or strains dependent on body position. Our purpose was to examine transpulmonary pressure (P_TP) and ...

Authors: Gustavo A Cortes-Puentes, Kenneth Gard, Joseph C Keenan, Alexander Adams, David Dries and John J Marini

Adrenomedullin: its double-edged sword during sepsis slices yet again

Authors: Matthijs Kox and Peter Pickkers

Effects of the PPAR-β/δ agonist GW0742 during resuscitated porcine septic shock

In un-resuscitated rodent models of septic shock, the peroxisome proliferator-activated receptor-β/δ (PPAR-β/δ) agonist GW0742 improved visceral organ function. Therefore, we tested the hypothesis whether GW07...

Authors: Martin Wepler, Sebastian Hafner, Angelika Scheuerle, Matthias Reize, Michael Gröger, Florian Wagner, Florian Simon, José Matallo, Frank Gottschalch, Andrea Seifritz, Bettina Stahl, Martin Matejovic, Amar Kapoor, Peter Möller, Enrico Calzia, Michael Georgieff…

IL-1β processing in mechanical ventilation-induced inflammation is dependent on neutrophil factors rather than caspase-1

Mechanical ventilation can cause ventilator-induced lung injury, characterized by a sterile inflammatory response in the lungs resulting in tissue damage and respiratory failure. The cytokine interleukin-1β (I...

Authors: Kim Timmermans, Selina EI van der Wal, Michiel Vaneker, Jeroen AWM van der Laak, Mihai G Netea, Peter Pickkers, Gert Jan Scheffer, Leo AB Joosten and Matthijs Kox

A mouse is not a rat is not a man: species-specific metabolic responses to sepsis - a nail in the coffin of murine models for critical care research?

Authors: Peter Radermacher and Philippe Haouzi

The metabolic phenotype of rodent sepsis: cause for concern?

Rodent models of sepsis are frequently used to investigate pathophysiological mechanisms and to evaluate putative therapeutic strategies. However, preclinical efficacy in these models has failed to translate t...

Authors: Parjam S Zolfaghari, Bernardo Bollen Pinto, Alex Dyson and Mervyn Singer

Activated protein C ameliorates impaired renal microvascular oxygenation and sodium reabsorption in endotoxemic rats

We aimed to test whether continuous recombinant human activated protein C (APC) administration would be able to protect renal oxygenation and function during endotoxemia in order to provide more insight into t...

Authors: Emre Almac, Tanja Johannes, Rick Bezemer, Egbert G Mik, Klaus E Unertl, AB Johan Groeneveld and Can Ince

Acid–base changes after fluid bolus: sodium chloride vs. sodium octanoate

This study aims to test the hypothesis that fluid loading with sodium chloride (150 mmol Na and 150 mmol Cl) or sodium octanoate (150 mmol Na, 100 mmol Cl, and 50 mmol octanoate) would lead to different acid–b...

Authors: Lu Ke, Paolo Calzavacca, Michael Bailey, Wei-qin Li, Rinaldo Bellomo and Clive N May

Epitope specificity of anti-Adrenomedullin antibodies determines efficacy of mortality reduction in a cecal ligation and puncture mouse model

Adrenomedullin (ADM), a circulating vasodilatory peptide, plays an important role in the development of sepsis-associated hemodynamic and microcirculatory disorders. While administration of exogenous ADM had b...

Authors: Joachim Struck, Frauke Hein, Siegmund Karasch and Andreas Bergmann

Adrenomedullin binding improves catecholamine responsiveness and kidney function in resuscitated murine septic shock

Adrenomedullin (ADM) has been referred to as a double-edged sword during septic shock: On one hand, ADM supplementation improved organ perfusion and function, attenuated systemic inflammation, and ultimately redu...

Authors: Katja Wagner, Ulrich Wachter, Josef A Vogt, Angelika Scheuerle, Oscar McCook, Sandra Weber, Michael Gröger, Bettina Stahl, Michael Georgieff, Peter Möller, Andreas Bergmann, Frauke Hein, Enrico Calzia, Peter Radermacher and Florian Wagner

A new dawn for critical care research

Authors: Mervyn Singer, Elie Azoulay and Jean-Daniel Chiche