Figure 3From: Early brain injury linearly correlates with reduction in cerebral perfusion pressure during the hyperacute phase of subarachnoid hemorrhageCells with DNA damage in hippocampus and basal cortex. Bar graphs demonstrate mean TUNEL-positive cell count of both hemispheres (±SD) subdivided into controls (n = 5) and different degrees of CPP depletion (mild, n = 5; moderate, n = 3; severe, n = 4). Quantification was performed by counting TUNEL-positive cells co-localizing with DAPI (blue) in regions of interest in the hippocampus (A) and basal cortex (B). Histochemistry (C): Co-localization with DAPI (left column) confirmed that TUNEL-positive staining (middle column) was generally in the nucleus (right column). Hollow arrows show DAPI-positive nuclear staining (blue). Filled arrows identify TUNEL-positive cells. Scale bars = 50 μm. *p < 0.05. **p < 0.01.Back to article page