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Intensive Care Medicine Experimental
Fig. 3 | Intensive Care Medicine Experimental

Fig. 3

From: Gentisic acid sodium salt, a phenolic compound, is superior to norepinephrine in reversing cardiovascular collapse, hepatic mitochondrial dysfunction and lactic acidemia in Pseudomonas aeruginosa septic shock in dogs

Fig. 3

Oxygen consumption rates (OCR) were measured in the Seahorse Instrument and examples are shown from mitochondria harvested from an animal in the septic control group (panel a) in a non-septic control dog (panel b) in the norepinephrine (NE) septic group (panel c) and in the gentisic septic group (panel d) under different metabolic conditions. In each experiment, we obtained two measurements in each condition and the average of the two conditions was obtained. In panel a basal OCR was slightly low in the animal in the septic control group as compared with the non septic control group. When adenosine diphosphate (ADP) was added to the preparation, OCR rate in the septic animal in panel A was not different from the animal in the non-septic control group (panel b). However, the response to FCCP (carbonyl cyanide p-triflouromethoxy-phenylhydrazone) in the animal in the septic control group (panel a) was attenuated as compared with the animal in the non-septic control group (panel b). With norepinephrine treatment, there was a restoration in the response to FCCP in the NE septic group (panel c). In the animal in the gentisic septic group, there was a large response in OCR to ADP which nearly equaled the response to FCCP (panel d)

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