Fig. 4

The endothelial bioreactor (EBR) system modulated pulmonary endothelial function. a The mRNA expression of CXCL1 and CXCL2 in the lungs was analyzed by quantitative real-time RT-PCR at 48 h after CLP (n = 7–9 per group). b The level of ICAM-1 in the lungs was examined by Western blot analysis at 48 h after CLP (n = 7–9 per group). c The mRNA expression of ICAM-1 in the lungs was analyzed by quantitative real-time RT-PCR at 48 h after CLP (n = 7–9 per group). d–f ICAM-1, CXCL1, and CXCL2 level of pulmonary endothelial cells from rats with or without CLP (n = 6 per group). g–i The EBR system was used in vitro, the UF of this system was used to stimulate pulmonary endothelial cells and the cells and supernatants were collected at 24 h after different stimuli (n = 6 per group). d, g The level of ICAM-1 in pulmonary endothelial cells was examined by Western blot analysis. e, h The mRNA expression of ICAM-1 in pulmonary endothelial cells was analyzed by quantitative real-time RT-PCR. f, i The levels of CXCL1 and CXCL2 in the culture supernatant of pulmonary endothelial cells were measured by ELISA. *p < 0.05, **p < 0.01, ***p < 0.001. Data are expressed as mean ± SEM. EBR endothelial bioreactor, CLP cecal ligation and puncture, UF ultrafiltration