Fig. 5
From: Endothelial bioreactor system ameliorates multiple organ dysfunction in septic rats
The endothelial bioreactor (EBR) system modulated the activation of peripheral blood neutrophils in vitro. a–d Peripheral blood neutrophils from rats with CLP were stimulated with culture supernatant of HUVECs for 4 h, and the supernatant were collected at 24 h after this stimulation. a, b The mean fluorescence intensity of CD11b was analyzed by flow cytometer (n = 7–9 per group). c, d The mean fluorescence intensity of intracellular free calcium was analyzed by flow cytometer (n = 7–9 per group). *p < 0.05, **p < 0.01, ***p < 0.001. Data are expressed as mean ± SEM. EBR endothelial bioreactor, CLP cecal ligation and puncture, MFI mean fluorescence intensity, HUVECs human umbilical vein endothelial cells