Fig. 4

Local and systemic injury and inflammation. Hematoxylin and eosin (H&E) staining of paraffin-embedded lung tissue sections of SI + 48 h (t = − 0.5 h) (A) and OMB treatment (t = 3 h) (B) animals (scale bar = 100 µm). Comparison of lung injury score (C) and lung wet/dry ratios (D) between SI + 48 h (t = − 0.5 h) and OMB treatment (t = 3 h) samples. E, F Immunoblot analysis of IL-1β expression levels in fresh frozen lung tissues of baseline (t = − 48 h), NT and OMB samples (t = 3 h). Difference between baseline and NT groups was statistically significant (p = 0.0092). G IL-6 marker analysis for NT and OMB (t = 3 h) for BAL and plasma samples. H–J Proteomic analysis of control (t = − 48 h), SI (NT, t = 3 h) and SI + OMB (t = 3 h) groups for their global protein expression as described in Materials and Methods section. Venn diagram showed 320 proteins with significant differential expression between SI and control groups (H, I). Heat map analysis of 68 proteins that were significantly upregulated or downregulated at 3 h post-OMB treatment (J). A p value of < 0.05 was considered statistically significant