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Intensive Care Medicine Experimental
Fig. 6 | Intensive Care Medicine Experimental

Fig. 6

From: Comparison of whole blood cytokine immunoassays for rapid, functional immune phenotyping in critically ill patients with sepsis

Fig. 6

IFNγ and TNF concentrations measured by ELLA immunoassay, following ex vivo exposure of whole blood to immune stimulants. A represents log2-transformed IFNγ and TNF concentrations, while B represents corresponding cytokine concentrations when normalized to number of cytokine-producing cells (lymphocytes or monocytes). Bars represent mean with 95% confidence interval. LPS lipopolysaccharide, CD3/CD28  anti-CD3 and anti-CD28 antibodies, PMA/iono  phorbol 12-myristate 13-acetate and ionomycin. n = 10 healthy volunteers, 24 survivors, 8 non-survivors (7 critically ill and septic and 1 critically ill and non-septic). *P ≤ 0.05, **P ≤ 0.01, based on two-tailed t test of log2-transformed cytokine concentrations from survivors versus non-survivors

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