Fig. 2
NVC clearance rapidly restores baseline spontaneous activity. A Representative voltage tracers for recordings of spontaneous action potentials. Blue tracers represent control and purple NVC clearance (NVCC). Shaded area represents application of Ca0.2. B Violin plots showing distribution of total AP count at 100 s in Ca1.1 and Ca0.2 in control and NVCC, schema similar to Fig. 1B. Mean AP counts in control were 35 ± 15 in Ca1.1, increasing to 670 ± 170 in Ca0.2. AP counts following NVCC in Ca1.1 and 92.4 ± 66 and 159.1 ± 37.7 in Ca0.2. Two-way RM ANOVA did not indicate an interaction between divalent change and NVCc (P = 0.063) although decreasing divalents had an overall effect (P = 0.024). While change in divalents increased spontaneous AP firing in control (P = 0.0013), there was no similar increase noted following NVCc (P = 0.99, N = 24 and 8, for control and NVCc, respectively). C Plots with individual values and mean of RMP. Blue denotes control and purple, NVCc. Individual values represented by solid circles (Ca1.1) and open circles (Ca0.2). Solid diamonds represent mean values. Mean ± SE for RMP in control; Ca1.1 = −73.1 ± 0.9 mV and Ca0.2 = −68.0 ± 1.4 mV, in NVCc; Ca1.1 = -65.7 ± 1.3 mV and Ca0.2 = −62.8 ± 1.5 mV. Two-way RM ANOVA indicates that divalent levels and NVCc had no interaction (F (1,93) = 2.578, P = 0.112), but both NVCc (P = 0.0004) and divalent reduction (P < 0.0001) had independent effects. Post hoc testing with Sidak multiple comparisons reveals that RMP was depolarized by NVCc at both Ca1.1 (P = 0.0002) and Ca0.2 (P = 0.009). N = 49 and 46 for control and NVCc, respectively