Fig. 4
Evoked divalent-dependent excitability remains attenuated after NVC clearance. A Exemplar traces showing AP firing following incremental 1-s current injections of 10–40 pA from RMP. Blue represents control and purple NVC clearance (NVCc). Dark tracers represent Ca1.1 and light Ca0.2. B Graphs showing average AP count in control (blue) and NVCc (purple), measurements in Ca1.1 (solid, filled circles) and Ca0.2 (faint, open circles) represented by separate lines. Error bars represent standard error. 40 pA current injections in control elicited 5.4 ± 1.5 APs in Ca1.1 and 10.9 ± 1.3 APs in Ca0.2 whereas NVCc elicited 6.6 ± 1.5 APs in Ca1.1 and 6.0 ± 1.3 APs in Ca0.2. C Plot showing average cumulative AP count for each recording. Schema similar to Fig. 3C. Control Ca1.1 and Ca0.2 = 12.9 ± 4.0 and 28.9 ± 4.4, whereas NVCc Ca1.1 and Ca0.2 = 18.1 ± 4.4 and 17.61 ± 3.4, respectively. Two-way RM ANOVA showing an interaction between divalent concentration and NVCc (F (1,66) = 7.820, P = 0.006). Simple main effects analysis showed there was no independent effect of NVCc (P = 0.544) but divalent concentration had an independent effect (P = 0.010). Post hoc analysis by Sidak multiple comparisons confirmed divalent-dependent increase in excitability was present in control (P = 0.003) but not in NVCc (P = 0.99). N = 37 and 31 for control and NVCc groups