Fig. 5
NVC transiently modifies AP threshold. A Plot of AP threshold, color schema similar to Fig. 1C. Mean ± SE values for control Ca1.1 vs. Ca0.2 = −49.3 ± 1.7 mV vs. −56.6 ± 1.5 mV and NVC Ca1.1 vs. Ca0.2 = −54.8 ± 1.7 mV vs. −56.1 ± 1.9 mV. Two-way RM ANOVA indicates that both divalent levels and NVC interact to hyperpolarize the AP threshold (F (1,25) = 5.99, P = 0.022). Post hoc testing with Sidak multiple comparisons shows hyperpolarization of AP threshold at Ca1.1 between control and NVC (P = 0.042), but this effect is absent at Ca0.2 (P = 0.835). In addition, divalent change hyperpolarized AP threshold in control (P < 0.001) but not NVC cells (P = 0.481). B Plot of AP threshold, color schema similar to Fig. 2C. Mean ± SE values for control Ca1.1 vs. Ca0.2 = −45.3 ± 1.3 mV vs. −47.6 ± 1.3 mV and NVCc Ca1.1 vs. Ca0.2 = −44.6 ± 1.5 mV vs. −46.3 ± 1.6 mV. Two-way RM ANOVA indicates that divalent levels and NVCc do not interact to change AP threshold (F (1,52) = 0.667, P = 0.418), but divalent change had an independent effect (P < 0.001). Post hoc testing with Sidak multiple comparisons shows divalent change hyperpolarized AP threshold in both control (P = 0.004) and NVCc cells (P = 0.041)