Volume 2 Supplement 1

ESICM LIVES 2014

Open Access

0101. Early and severe impairment of lactate clearance in endotoxic shock is not related to liver hypoperfusion: preliminary report

  • P Tapia1,
  • D Soto1,
  • A Bruhn1,
  • T Regueira1,
  • N Jarufe2,
  • L Alegria1,
  • JP Bachler2,
  • F Leon2,
  • C Vicuña1,
  • C Luengo3,
  • G Ospina-Tascón4,
  • J Bakker5 and
  • G Hernandez1
Intensive Care Medicine Experimental20142(Suppl 1):P12

DOI: 10.1186/2197-425X-2-S1-P12

Published: 26 September 2014

Introduction

Although the prognostic value of persistent hyperlactatemia in septic shock is unequivocal, its physiological determinants are controversial. In particular, the role of impaired hepatic clearance has been considered as relevant only in severe shock with liver ischemia or advanced cirrhosis. However, very few studies have addressed this subject.

Objectives

To determine the evolution of lactate clearance [1] in an endotoxic sheep model.

Methods

This study is part of a major project exploring the influence of adrenergic stimulation and blockade over the determinants of lactate production and utilization in septic shock. Eight anesthetized sheep subjected to a multimodal hemodynamic/perfusion assessment including pulmonary artery, hepatic and portal vein catheterizations, portal/hepatic artery flow, gut tonometry, sublingual microcirculation, muscle microdialysis and hepatic mitochondrial high-resolution respirometry, were randomized to LPS or sham. LPS sheep received 5 mcg/kg bolus (E coli O127:B8®) and then 4 mcg •kg-1•hr-1 for the rest of the experiment [2]. After 1h they were volume resuscitated. Sampling and exogenous lactate clearances were performed at 4 points (fig 1)
https://static-content.springer.com/image/art%3A10.1186%2F2197-425X-2-S1-P12/MediaObjects/40635_2014_Article_69_Fig1_HTML.jpg

Figure 1

Results

LPS sheep presented an early hyperlactatemic hyperdynamic septic shock and an increased muscle lactate production compared to sham (MAP 63 vs 87 mmHg; MPAP 20 vs 10 mmHg; CO 3.7 vs 2.5 l/min; arterial lactate 6.1 vs 2.6 mmol/l). Total liver flow (865 vs 692 ml/min), proportion of liver flow/CO, O2 liver extraction, liver enzymes, and mitochondrial function were comparable between LPS and placebo (fig 2)
https://static-content.springer.com/image/art%3A10.1186%2F2197-425X-2-S1-P12/MediaObjects/40635_2014_Article_69_Fig2_HTML.jpg

Figure 2

However, LPS sheep presented an early severe and persistent decrease in lactate clearance (C: 575 vs 1188 and D: 907 vs 1410 ml/kg/h), fig 3
https://static-content.springer.com/image/art%3A10.1186%2F2197-425X-2-S1-P12/MediaObjects/40635_2014_Article_69_Fig3_HTML.jpg

Figure 3

Conclusions

Hyperdynamic endotoxic shock induces an early, severe and persistent impaired lactate clearance that is not related to liver hypoerfusion, O2 extraction or mitochondrial function.

Declarations

Grant acknowledgment

FONDECYT 1130200, Chile.

Authors’ Affiliations

(1)
Pontificia Universidad Católica de Chile, Facultad de Medicina, Departamento de Medicina Intensiva
(2)
Pontificia Universidad Católica de Chile, Facultad de Medicina, Departamento de Cirugía Digestiva
(3)
Universidad de Chile, Hospital Clínico, Unidad de Pacientes Críticos
(4)
Fundación Valle del Lili, Intensive Care Unit
(5)
Erasmus MC University Medical Centre, Department of Intensive Care Adults

References

  1. Levraut J, et al.: Am J Respir Crit Care Mer. 1998, 157: 1021–1026. 10.1164/ajrccm.157.4.9705037View ArticleGoogle Scholar
  2. Dubin A, et al.: Crit Care Med. 2008, 36: 535–542. 10.1097/01.CCM.0000300083.74726.43PubMedView ArticleGoogle Scholar

Copyright

© Tapia et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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