Epitope specificity of anti-Adrenomedullin antibodies determines efficacy of mortality reduction in a cecal ligation and puncture mouse model

Intensive Care Medicine Experimental

Table 1 Overview on monoclonal anti-ADM antibodies and their characteristics

Antibody #	Immunogen	Amino acid position of immunogen in adrenomedullin	IgG subtype	Kd (M)	Maximal inhibition in bioassay (%)
NT-M	YRQSMNQGSRSNGCRFGTC	1-19	2a	5.8 × 10^-9	24.0
MR-M	CTFQKLAHQIYQ	19-31	1	5.9 × 10^-9	61.7
CT-M	CAPRNKISPQGY-NH₂	Cys-40-50	2b	9.0 × 10^-9	102.1
NT-H	YRQSMNNFQGLRSFGCRFGTC	1-21	1	1.6 × 10^-8	25.5
MR-H	CTVQKLAHQIYQ	21-32	1	2.0 × 10^-9	74.4
CT-H	CAPRSKISPQGY-NH₂	Cys-42-52	1	1.1 × 10^-9	100.7

The antibodies were named according to their epitope specificities as follows: NT, anti-N-terminal region; MR, anti-midregion; CT, anti-C-terminal region; and the suffixes M and H designate mouse and human ADM, respectively.