Volume 2 Supplement 1

ESICM LIVES 2014

Open Access

0897. Mechanisms of pulmonary inflation during lung injury assessed by synchrotron radiation computed tomography

  • G Perchiazzi1,
  • JB Borges2,
  • G Hedenstierna2,
  • L Porra3,
  • L Broche4,
  • M Pellegrini1, 2,
  • A Sindaco1,
  • AP Tannoia1,
  • S Derosa1,
  • FF Todisco1,
  • T Fiore1,
  • A Larsson2 and
  • S Bayat5
Intensive Care Medicine Experimental20142(Suppl 1):O23

https://doi.org/10.1186/2197-425X-2-S1-O23

Published: 26 September 2014

Introduction

The process of lung inflation during mechanical ventilation is characterized by phenomena of alveolar recruitment and distension but regional interaction and temporal sequence are not known for the core areas of the lung. The only information available are referred to subpleural alveoli, studied by microscopy. Relevance of this issue derives from the notion that 1) radiologic studies reveal that air spaces behavior in the core regions of the parenchyma is very complex 2) unsuited patterns of mechanical ventilation (MV) can trigger a Ventilator-Induced Lung Injury (VILI). Synchrotron Radiation Computed Tomography (SRCT) yields tomographic images at resolutions higher than conventional CT.

Objectives

The aim of this study was to evaluate the behavior of air spaces using SRCT in an animal model of VILI at different Positive End Expiratory Pressure (PEEP) levels.

Methods

Six anesthetized, tracheostomized rabbits were mechanically ventilated to preserve healthy conditions (HC) of the lung (Tidal Volume=7ml/kg, PEEP=3 cmH2O) and a sequence of SRCT exposures was performed at 40 different consecutive gravitational levels, at PEEP levels of 12, 9, 6, 3, 0 cmH2O, during end-expiratory pauses. Then VILI was induced combining repeated lung lavages followed by injurious MV in Pressure Control mode with Peak Pressure=35 cmH20 and ZEEP, for one hour; then the same sequence of SRCT exposures was performed. SRCT images (resolution = 45.5 µm) were transformed into binary images. From each animal, from a stack of 40 images at different gravitational levels, a Region Of Interest (ROI) of 100x200 voxels was studied by using the Image Processing Toolbox for Matlab. The increase of air spaces numerosity (NA) was considered as an index of alveolar recruitment while the increase of air spaces area (SA) and perimeter (PE) as indices of alveolar expansion. The statistical analysis was performed applying the Student's T test (a=0.05) on NA, SA and PE during HC and VILI at different PEEP levels.

Results

During healthy conditions (HC), SA and PE increased in direct proportion to PEEP up to 9 cmH2O, then remained stable; NA increased up to PEEP=6 cmH2O and then decreased with higher PEEP values. During VILI, SA, PE and NA increased with PEEP in a uniform manner.

Conclusions

In HC the changes of shape and size of air spaces seemed to provide a greater contribution to the increase of lung volume than recruitment mechanisms. NA tendency to decrease after a peak was interpreted as a consequence of progressive stretching of interstitial septa with creation of false connections between adjacent air spaces (false derecruitment). In VILI, alveolar recruitment during lung inflation was a continuous pattern throughout the range of PEEP levels that were studied.

Declarations

Grant acknowledgment

The School of Anesthesia and Intensive Care of Medicine, Bari University; The Swedish Research Council; The Swedish Heart Lung Fund; The European Synchrotron Radiation Facility.

Authors’ Affiliations

(1)
Bari University, Emergency and Organ Transplant
(2)
Uppsala University, Dept of Medical and Surgical Sciences
(3)
University of Helsinki, Dept of Physics
(4)
European Synchrotron Radiation Facility
(5)
Université de Picardie Jules Verne

Copyright

© Perchiazzi et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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