Volume 2 Supplement 1


Open Access

0104. Modulatory effects of heat shock with or without glutamine compared to LPS on peripheral blood mononuclear cells heat-shock-protein 90α expression in severe sepsis and trauma

  • E Briassouli1,
  • M Tzanoudaki2,
  • G Daikos1,
  • K Vardas3,
  • M Kanariou2,
  • C Routsi3,
  • S Nanas3 and
  • G Briassoulis4
Intensive Care Medicine Experimental20142(Suppl 1):P14


Published: 26 September 2014


Inflammatory stimuli cause posttranslational modifications of inducible 90α-kDa-heat-shock-protein (HSP90α) that are Hsp90-inhibitor sensitive and may be important to the pro-inflammatory actions of Hsp90α.


We investigated the heat-shock (HS) and lipopolysaccharide (LPS)-stress response effect on HSP90α in cultured peripheral blood mononuclear cells (PBMCs) from patients with severe sepsis (SS) or trauma-related systemic inflammatory response syndrome (SIRS) compared to healthy-controls (H) and any possible modulating Glutamine (Gln)-effect.


PBMCs of 16/H, 11/SS, and 7/SIRS were incubated with 1µg/ml LPS or 43oHS vs.no stimulation for 4h. In each group 3 experiments involved L-Ala-Gln10mM incubation 1h before (Gln-b) or after (Gln-a) induction, or no glutmanine (1088 measurements). Intracellular Mean Fluorescence Intensity (MFI) levels of monocytes (mHSP90α) or lymphocytes (lHSP90α) determined using Flow Cytometry.


Baseline mHSP90α was higher in SIRS (187±30 vs. 112±10, p< 0.01) and lHSP90α in SS (91±19 vs. 47±3, p< 0.001) compared to H. LPS induced H-mHSP90α (141±12 vs. 112±10, p< 0.001) and HS H-lHSP90α (66±7 vs. 47±3, p< 0.0001). Neither LPS nor HS exhibit any significant effect in SIRS- or SS-mHSP90α or lHSP90α. Glutamine given before LPS suppressed SS-lHSP90α (Gln-b 61±5 vs. 91±19, p< 0.004). Similarly, when glutamine was given before or after HS suppressed SS-lHSP90α (Gln-a 73±5 vs. 91±19, p< 0.001; Gln-b 78±4 vs. 91±19, p< 0.05), respectively.


PBMCs express higher baseline mHSP90α in SIRS and lHSP90α in SS, not further induced by LPS or HS, contrasting their induction effects in H. Gln pre-treatment may attenuate the LPS or HS-induced lHSP90α in SS.


Grant acknowledgment

This research has been co-financed by the European Union (European Social Fund (ESF)) and Greek national funds through the Operational Program ''Education and Lifelong Learning'' of the National Strategic Reference Framework (NSRF)-Research Funding Program: THALES.

Authors’ Affiliations

1st Department of Propaedeutic InternalMedicine, University of Athens, Athens, Greece
Department of Immunology - Histocompatibility, Specialized Center & Referral Center for Primary Immunodeficiencies - Paediatric Immunology, “Aghia Sophia” Children's Hospital, Athens, Greece
First Critical Care Department, University of Athens, Evangelismos Hospital, Athens, Greece
PICU, University of Crete, University Hospital, Heraklion, Greece


© Briassouli et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.