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Volume 2 Supplement 1

ESICM LIVES 2014

0104. Modulatory effects of heat shock with or without glutamine compared to LPS on peripheral blood mononuclear cells heat-shock-protein 90α expression in severe sepsis and trauma

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Introduction

Inflammatory stimuli cause posttranslational modifications of inducible 90α-kDa-heat-shock-protein (HSP90α) that are Hsp90-inhibitor sensitive and may be important to the pro-inflammatory actions of Hsp90α.

Objectives

We investigated the heat-shock (HS) and lipopolysaccharide (LPS)-stress response effect on HSP90α in cultured peripheral blood mononuclear cells (PBMCs) from patients with severe sepsis (SS) or trauma-related systemic inflammatory response syndrome (SIRS) compared to healthy-controls (H) and any possible modulating Glutamine (Gln)-effect.

Methods

PBMCs of 16/H, 11/SS, and 7/SIRS were incubated with 1µg/ml LPS or 43oHS vs.no stimulation for 4h. In each group 3 experiments involved L-Ala-Gln10mM incubation 1h before (Gln-b) or after (Gln-a) induction, or no glutmanine (1088 measurements). Intracellular Mean Fluorescence Intensity (MFI) levels of monocytes (mHSP90α) or lymphocytes (lHSP90α) determined using Flow Cytometry.

Results

Baseline mHSP90α was higher in SIRS (187±30 vs. 112±10, p< 0.01) and lHSP90α in SS (91±19 vs. 47±3, p< 0.001) compared to H. LPS induced H-mHSP90α (141±12 vs. 112±10, p< 0.001) and HS H-lHSP90α (66±7 vs. 47±3, p< 0.0001). Neither LPS nor HS exhibit any significant effect in SIRS- or SS-mHSP90α or lHSP90α. Glutamine given before LPS suppressed SS-lHSP90α (Gln-b 61±5 vs. 91±19, p< 0.004). Similarly, when glutamine was given before or after HS suppressed SS-lHSP90α (Gln-a 73±5 vs. 91±19, p< 0.001; Gln-b 78±4 vs. 91±19, p< 0.05), respectively.

Conclusions

PBMCs express higher baseline mHSP90α in SIRS and lHSP90α in SS, not further induced by LPS or HS, contrasting their induction effects in H. Gln pre-treatment may attenuate the LPS or HS-induced lHSP90α in SS.

Grant acknowledgment

This research has been co-financed by the European Union (European Social Fund (ESF)) and Greek national funds through the Operational Program ''Education and Lifelong Learning'' of the National Strategic Reference Framework (NSRF)-Research Funding Program: THALES.

Author information

Correspondence to E Briassouli.

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Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0), which permits use, duplication, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Briassouli, E., Tzanoudaki, M., Daikos, G. et al. 0104. Modulatory effects of heat shock with or without glutamine compared to LPS on peripheral blood mononuclear cells heat-shock-protein 90α expression in severe sepsis and trauma. ICMx 2, P14 (2014) doi:10.1186/2197-425X-2-S1-P14

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Keywords

  • Public Health
  • Flow Cytometry
  • Fluorescence Intensity
  • Stress Response
  • Glutamine