- Poster presentation
- Open Access
0104. Modulatory effects of heat shock with or without glutamine compared to LPS on peripheral blood mononuclear cells heat-shock-protein 90α expression in severe sepsis and trauma
Intensive Care Medicine Experimental volume 2, Article number: P14 (2014)
Inflammatory stimuli cause posttranslational modifications of inducible 90α-kDa-heat-shock-protein (HSP90α) that are Hsp90-inhibitor sensitive and may be important to the pro-inflammatory actions of Hsp90α.
We investigated the heat-shock (HS) and lipopolysaccharide (LPS)-stress response effect on HSP90α in cultured peripheral blood mononuclear cells (PBMCs) from patients with severe sepsis (SS) or trauma-related systemic inflammatory response syndrome (SIRS) compared to healthy-controls (H) and any possible modulating Glutamine (Gln)-effect.
PBMCs of 16/H, 11/SS, and 7/SIRS were incubated with 1µg/ml LPS or 43oHS vs.no stimulation for 4h. In each group 3 experiments involved L-Ala-Gln10mM incubation 1h before (Gln-b) or after (Gln-a) induction, or no glutmanine (1088 measurements). Intracellular Mean Fluorescence Intensity (MFI) levels of monocytes (mHSP90α) or lymphocytes (lHSP90α) determined using Flow Cytometry.
Baseline mHSP90α was higher in SIRS (187±30 vs. 112±10, p< 0.01) and lHSP90α in SS (91±19 vs. 47±3, p< 0.001) compared to H. LPS induced H-mHSP90α (141±12 vs. 112±10, p< 0.001) and HS H-lHSP90α (66±7 vs. 47±3, p< 0.0001). Neither LPS nor HS exhibit any significant effect in SIRS- or SS-mHSP90α or lHSP90α. Glutamine given before LPS suppressed SS-lHSP90α (Gln-b 61±5 vs. 91±19, p< 0.004). Similarly, when glutamine was given before or after HS suppressed SS-lHSP90α (Gln-a 73±5 vs. 91±19, p< 0.001; Gln-b 78±4 vs. 91±19, p< 0.05), respectively.
PBMCs express higher baseline mHSP90α in SIRS and lHSP90α in SS, not further induced by LPS or HS, contrasting their induction effects in H. Gln pre-treatment may attenuate the LPS or HS-induced lHSP90α in SS.
This research has been co-financed by the European Union (European Social Fund (ESF)) and Greek national funds through the Operational Program ''Education and Lifelong Learning'' of the National Strategic Reference Framework (NSRF)-Research Funding Program: THALES.
About this article
Cite this article
Briassouli, E., Tzanoudaki, M., Daikos, G. et al. 0104. Modulatory effects of heat shock with or without glutamine compared to LPS on peripheral blood mononuclear cells heat-shock-protein 90α expression in severe sepsis and trauma. ICMx 2, P14 (2014) doi:10.1186/2197-425X-2-S1-P14
- Public Health
- Flow Cytometry
- Fluorescence Intensity
- Stress Response