Volume 2 Supplement 1

ESICM LIVES 2014

Open Access

0854. Metabolomic changes by mass spectrometry in lung tissue from septic rats with mechanical ventilation-induced lung injury

  • Y Rojas1, 2,
  • S Naz3,
  • N Nin4, 5,
  • A Garcìa3,
  • A Ferruelo1, 2,
  • L Martínez-Caro1, 2,
  • M de Paula1, 6,
  • C Barbas3 and
  • JA Lorente1, 2, 7
Intensive Care Medicine Experimental20142(Suppl 1):P63

https://doi.org/10.1186/2197-425X-2-S1-P63

Published: 26 September 2014

Objective

To identify metabolomic changes in lung tissue associated with lung injury induced by mechanical ventilation (VILI) in animals with sepsis, using for the first time a global unbiased metabolomic fingerprinting approach.

Methods

Rats received cecal-ligation and puncture (CLP) or sham operation, and 24 h later underwent mechanical ventilation for 2.5 h with either VT=9 ml/kg, positive end-expiratory pressure (PEEP)=0 cm H2O (n=9 and n=12, without and with CLP, respectively); or VT=25 ml/kg, PEEP=5 cm H2O (n=13 and n=12, without and with CLP, respectively). Lung tissue samples were obtained and analyzed by nontargeted global fingerprinting approach for lung tissue analysis, applying multiple complementary analytical techniques, including liquid cromatography-mass spectrometry (MS), gas cromatography-MS, and capillary electrophoresis-MS. We followed the Principles of Laboratory Animal Care (2010/63/UE 22-09, RD 53/2013 BOE 1-02, ley 32/2007 BOE 7-11).

Results

Metabolomic changes characteristic of sepsis and VILI were identified. Lung tissue samples from septic rats with VILI were characterized by a specific metabolomic profile as compared to samples from septic rats without VILI. Metabolomic changes indicated increased oxidative stress, and changes in purine, energy, carnitine, aminoacid, urea cycle, vitamines, collagen, ceramide-sphingomyelin and phospholipid metabolism.

Conclusion

A particular metabolomic profile can be identified in lung tissue from septic rats with lung injury induced by mechanical ventilation.

Declarations

Grant acknowledgment

FIS 12/02898, FIS 11/02791, FIS 12/02451, European Network (7th FP) ITN 264864, CA11/00260.

Authors’ Affiliations

(1)
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Getafe, Spain
(2)
Intensive Care Service, Hospital Universitario de Getafe, Getafe, Spain
(3)
Facultad de Farmacia, Centro de Metabolómica y Bioanálisis (CEMBIO), Universidad CEU San Pablo, Madrid, Spain
(4)
Intensive Care Service, Hospital Español, Montevideo, Uruguay
(5)
Intensive Care Service, Hospital de Torrejon, Madrid, Spain
(6)
Hospital Universitario de Getafe, Getafe, Spain
(7)
Universidad Europea de Madrid, Madrid, Spain

Copyright

© Rojas et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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