Skip to main content

Volume 2 Supplement 1


0991. Nebulized heparin reduces pulmonary inflammatory responses in a rat model of acute lung injury


Sepsis is a mayor cause of acute lung injury (ALI). Pulmonary coagulopathy is intrinsic to ALI and in sepsis, anticoagulant system is impaired, due to consumption and downregulation by inflammatory mediators. Several studies, in ALI patients and in ALI experimental models, inconsistently suggest beneficial effects of systemic anticoagulants which affect systemic coagulation. Nebulization of anticoagulants might allow for higher pulmonary concentration and reduce the risk of systemic bleeding.


To assess the effects of local heparin treatment in a rat model of acute lung injury.


Adult male Sprague-Dawley rats (250-300 g; n=6/group) were anesthetized with isofluorane and subjected to intratracheal administration (IA) of LPS (10 µg/g b.w.). Saline or heparin (1000 IU/kg) were nebulized at 4 and 8h after LPS instillation. Animals were sacrificed 24h after the injury. Inflammatory cells and total proteins were assessed in bronchoalveaolar lavage fluid (BALF). IL-6, GRO-κC, TNF-α and IL-10 were measured in lung homogenate by multiplex assay (Luminex, Merck Millipore, Darmstadt, Germany). Data are reported as mean±SD. One-way ANOVA was used for multigroup comparisons.


In BALF, nebulized heparin significantly reduced neutrophils in animals instilled with LPS (12.4±4.3x107 cells/ml) compared to animals administrated with LPS and nebulized with saline (18.1±6.1x107 cells/ml, p< 0.05). Total BALF proteins were found to be lower in rats nebulized with heparin (578.3±89.8 µg/ml) than in rats treated with saline (914.6±250.1 µg/ml, p< 0.005). In lung homogenate, IL-6, TNF-α, GRO-κC levels significantly decreased in animals nebulized with heparin compared to those ones nebulized with saline (IL6: LPS+Sal: 147.7±1.3 ng/ml, LPS+Hep: 47.3±20.2 ng/ml, p< 0.005; TNF-α: LPS+Sal: 1.1±0.2 ng/ml, LPS+Hep: 0.3±0.1 ng/ml, p< 0.005; GRO-κC: LPS+Sal: 45.9±22.9 ng/ml, LPS+Hep: 40.9±9.8 ng/ml, p< 0.005). IL-10 did not show any significant difference among groups.


Our results show that local heparin administration reduces pulmonary inflammatory responses in a rat model of acute lung injury.

Grant acknowledgment

FIS-PI12/02548, CIBERES and Fundació Parc Taulí, AGAUR GRC 532.

Author information

Authors and Affiliations


Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits use, duplication, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chimenti, L., Guillamat, R., Gomez, M. et al. 0991. Nebulized heparin reduces pulmonary inflammatory responses in a rat model of acute lung injury. ICMx 2 (Suppl 1), P76 (2014).

Download citation

  • Published:

  • DOI: