Skip to main content

Volume 3 Supplement 1


Evaluation of early immune response in multitrauma patients admitted in ICU: the effect of age


It is known the negative effect of urgent clinical incidents on the immunological status of patients. Several clinical and demographic data, including age are related to it.


The aim of the study is the evaluation of early immune response in multitrauma patients on admission in ICU according to patient's age.


Thirty two multitrauma patients issued in ICU included in the study classified into two groups according to their age, (I) adults ≤65 (N = 14) and (II) elderly >65 (N = 18) years old. Ten milliliters of peripheral blood collected from each patient on admisssion, divided into two tubes with/without anticoagulant. Whole blood samples diluted 1:10 with RPMI 1X, incubated with /without 500pg/ml LPS at 37°C for 4H. Serum and cell culture supernatants (CCSPs) isolated by centrifugation and stored at -70°C. Tumour necrosis factor alpha (TNF-a) and interleukins 6, 8, 10 (IL-6, IL-, IL-10) measured in serum and CCSPs with ELISA. Statistical analysis was performed with Graphpad 5.0. Data are presented as Median +/- IQRs; a P value < 0.05 accepted as significant.


Serum TNF-a was significantly higher in elderly (P < 0.01), while IL-6, IL-8 and IL-10 had no significant differences between groups. Opposite, ex vivo cytokines release was significantly lower in elderly (I)compare to adults (II), TNF-a (P < 0.05), IL-6 (P < 0.01) and IL-8 (P < 0.01) but not IL-10 (P > 0.05).


The effect of age seems to be critical on the immune response of severe trauma patients at the early state of the incident.

Figure 1
figure 1

Patient's age in groups (I) adults ≤65 and (II) elderly >65.

Figure 2
figure 2

Serum cytokines levels in groups I and II. ***P < 0.001.

Figure 3
figure 3

Ex vivo LPS cytokines release in groups I and II, after baseline removal. *P < 0.0, **P < 0.01.


  1. Filippou L, Venetsanou K, Voulalas G, Markopoulou D, Chroni D, Maltezos C, Alamanos I: Study of the ex vivo immune response of polytrauma older patients in the ICU on admission: preliminary results. Critical Care. 2014, 18 (Suppl 1): P236-10.1186/cc13426.

    Article  PubMed Central  Google Scholar 

  2. Bruunsgaard H, Pedersen AN, ScholL M, kinhoz P, Pedersen BK: Impaired production of proinflammatory cytokines in response to lipopolysaccharide (LPS) stimulation in elderly humans. Clin Exp Immunol. 1999, 118: 235-241. 10.1046/j.1365-2249.1999.01045.x.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Open Access  This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.

The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

To view a copy of this licence, visit

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Markopoulou, D., Venetsanou, K., Kaldis, V. et al. Evaluation of early immune response in multitrauma patients admitted in ICU: the effect of age. ICMx 3 (Suppl 1), A372 (2015).

Download citation

  • Published:

  • DOI: