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Volume 3 Supplement 1


  • Poster presentation
  • Open Access

Impact of persistent endotracheal tube biofilm on ventilator-associated pneumonia clinical and microbiological response

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Intensive Care Medicine Experimental20153 (Suppl 1) :A700

  • Published:


  • Interquartile Range
  • Endotracheal Tube
  • Tobramycin
  • Colistin
  • Empiric Antibiotic Treatment


Ventilator-associated pneumonia (VAP) is the most common infectious complication in critically ill patients. The formation of biofilm on endotracheal tube (ETT) surface plays an important role in pathogenesis of this infection, because it facilitates the growth and survival of microorganisms and difficults the action of antimicrobial therapy.


The aim of this study is to evaluate the influence of the persistence of viable microorganisms in the ETT biofilm on clinical and microbiological response to VAP.


All patients over 18 years old admitted to the Intensive Care Unit of the Hospital Universitario and Politecnico la Fe (Valencia, Spain), undergoing mechanical ventilation and with VAP diagnosis were included. Clinical and microbiological data were collected at the moment of inclusion and on days 3 and 7 after VAP diagnosis. After ETT removal, ETT was washed and a piece of 0.5 cm length was cut and sonicated and the resulting material was quantitatively cultured (100 uL blood agar plate with counting of colonies with 100 uL in isolation in chocolate blood agar). Clinical response (resolution of VAP signs: fever, high leucocyte count and respiratory purulence) was evaluated at day 3 and day 7. Median and interquartile range (IR) was calculated for continuous variables, and absolute and relative frequencies for discrete variables. Statistical analysis was done by chi-square test.


23 patients with VAP diagnosis were included, 60% men, age 55 years (IR: 51 - 67), APACHE II 22 (IR: 15 - 25). The median of days of mechanical ventilation was 17 days (IR: 13 - 20) and until VAP diagnosis was 11 days (IR: 6 - 14). The most common etiological agents were gram-negative bacilli producers of extended-spectrum beta-lactamases (ESBL) (43.6%), multidrug resistant (MDR) P. aeruginosa (17.4%), MDR A. baumannii (17.4%) and methicillin-sensitive S. aureus (17.4%). 83% of patients received an appropriate empiric antibiotic treatment. Median SOFA scale at VAP diagnosis was 5 (IR: 3 - 8). EET biofilm (persistence of viable microorganisms after sample sonication) was detected in 61% of the cases. No differences were observed according to the appropriateness of initial empiric treatment (78.6% vs. 80%; p 0.728) or the use of inhaled antibiotics such as tobramycin or colistin (42.8% vs. 20%; p 0.366). ETT biofilm was associated with lower percentages of clinical response on days 3 (7% vs. 20 %; p 0.468) and day 7 (21.4% vs. 60%; p 0.176).


Persistence of viable and potentially infectious microorganisms on ETT biofilm is a common phenomenon after VAP and is not influenced by the use of systemic or inhaled antibiotics. Although our sample size does not allow drawing conclusions, the microbiological persistence seems to be associated with a worse VAP clinical response.

Authors’ Affiliations

Critical Care Medicine, Hospital Universitari i Politècnic La Fe, Valencia, Spain
Microbiology, Hospital Universitari i Politècnic La Fe, Valencia, Spain


  1. Fernández-Barat L, et al: FEMS Immunol Med Microbiol. 2012, 65 (2): 309-17. JulGoogle Scholar
  2. Gil-Perotin S et al: Crit Care. 2012, 16 (3): R93-10.1186/cc11357. May 23View ArticleGoogle Scholar


© Gordon Sahuquillo et al.; 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.