Figure 6
Effect of DNase treatment on pulmonary and hepatic inflammation after infection with B. pseudomallei. Representative hematoxylin and eosin stained slides of lung (A, B) and liver (D, E) of control and DNase-treated mice 72 h post-infection with B. pseudomallei (103 cfu). Marked inflammation was seen post-infection, and areas of necrosis (marked with a number sign) and thrombosis formation (marked with an asterisk) were seen in both groups. Significantly less liver inflammation was observed in the DNase-treated group when compared to controls 72 h after infection. Total organ pathology score (lung and liver (C, F)) was determined 24 and 72 h post-infection according to the scoring system as described [41],[39],[40]. Graphs depict eight mice per group and time point. Median values are shown, and statistical significance was determined using the unpaired Mann-Whitney U test. *P < 0.05.