Skip to main content

Table 1 Cytokines and markers for systemic organ injury do not differ between mice treated with intravenous DNase or PBS post-infection with B. pseudomallei

From: Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)

  24 h 72 h
Control DNase Pvalue Control DNase Pvalue
Plasma       
 TNF-α (pg/mL) 3.3 ± 2 4.1 ± 2 0.41 407 ± 319 328.3 ± 300 0.62
 MCP-1 (pg/mL) 11.7 ± 13 21.5 ± 8 0.09 3,976 ± 2,183 2,879 ± 1,937 0.31
 IL-12p70 (pg/mL) ND ND   8.6 ± 10 21.6 ± 20 0.13
 IL-6 (pg/mL) 115.3 ± 73 104 ± 62 0.74 8,022 ± 3,814 6,990 ± 4,252 0.62
 IFN-γ (pg/mL) 4.0 ± 6 4.8 ± 4 0.76 1,914 ± 1,152 1,791 ± 1,490 0.86
 IL-10 (pg/mL) ND ND   ND ND  
 AST (U/L) 63.6 ± 6 74.4 ± 37 0.83 356.6 ± 191 247 ± 143 0.50
 ALT (U/L) 21.7 ± 9 27 ± 24 0.92 155.4 ± 59 138 ± 98 0.60
 LDH (U/L) 210 ± 74 312.9 ± 157 0.21 1,115 ± 758 873.2 ± 375 0.71
 Urea (mmol/L) 9.8 ± 2 7.7 ± 2 0.04 15.9 ± 10 14.4 ± 5 0.91
 Creatinine (μmol/L) 11.3 ± 2 9.6 ± 4 0.43 15.7 ± 17 8.4 ± 7 0.53
Lung       
 TNF-α (pg/mL) 239.1 ± 110 427.8 ± 100 0.003 3,182 ± 1,440 3,261 ± 1,382 0.91
 IL-6 (pg/mL) 945.9 ± 348 1,103 ± 201 0.29 8,601 ± 3,329 7,521 ± 3,482 0.54
 IL-10 (pg/mL) 205.8 ± 147 266.4 ± 57 0.29 559.4 ± 219 498.3 ± 107 0.49
 CXCL1 (pg/mL) 6,771 ± 4,247 6,147 ± 2,284 0.72 26,421 ± 10,123 26,305 ± 10,450 >0.99
  1. Cytokines and markers for systemic organ injury were measured in plasma and/or lung homogenates 24 and 72 h post-infection. Data are means ± SD (cytokines) or median (range) of five to eight mice per group. Actual P values are shown, determined via t test or Mann-Whitney U where appropriate; ND, not detectable or below detection limit; TNF-α, tumor necrosis factor-alpha; MCP-1, monocyte chemo-attractive protein-1; IL, interleukin; IFN-γ, interferon-gamma; AST, aspartate transaminase; ALT, alanine transaminase; LDH, lactate dehydrogenase; CXCL1, chemokine (C-X-C motif) ligand 1.