Intensive Care Medicine Experimental

Table 1 Cytokines and markers for systemic organ injury do not differ between mice treated with intravenous DNase or PBS post-infection with B. pseudomallei

From: Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)

	24 h			72 h
	Control	DNase	Pvalue	Control	DNase	Pvalue
Plasma
TNF-α (pg/mL)	3.3 ± 2	4.1 ± 2	0.41	407 ± 319	328.3 ± 300	0.62
MCP-1 (pg/mL)	11.7 ± 13	21.5 ± 8	0.09	3,976 ± 2,183	2,879 ± 1,937	0.31
IL-12p70 (pg/mL)	ND	ND		8.6 ± 10	21.6 ± 20	0.13
IL-6 (pg/mL)	115.3 ± 73	104 ± 62	0.74	8,022 ± 3,814	6,990 ± 4,252	0.62
IFN-γ (pg/mL)	4.0 ± 6	4.8 ± 4	0.76	1,914 ± 1,152	1,791 ± 1,490	0.86
IL-10 (pg/mL)	ND	ND		ND	ND
AST (U/L)	63.6 ± 6	74.4 ± 37	0.83	356.6 ± 191	247 ± 143	0.50
ALT (U/L)	21.7 ± 9	27 ± 24	0.92	155.4 ± 59	138 ± 98	0.60
LDH (U/L)	210 ± 74	312.9 ± 157	0.21	1,115 ± 758	873.2 ± 375	0.71
Urea (mmol/L)	9.8 ± 2	7.7 ± 2	0.04	15.9 ± 10	14.4 ± 5	0.91
Creatinine (μmol/L)	11.3 ± 2	9.6 ± 4	0.43	15.7 ± 17	8.4 ± 7	0.53
Lung
TNF-α (pg/mL)	239.1 ± 110	427.8 ± 100	0.003	3,182 ± 1,440	3,261 ± 1,382	0.91
IL-6 (pg/mL)	945.9 ± 348	1,103 ± 201	0.29	8,601 ± 3,329	7,521 ± 3,482	0.54
IL-10 (pg/mL)	205.8 ± 147	266.4 ± 57	0.29	559.4 ± 219	498.3 ± 107	0.49
CXCL1 (pg/mL)	6,771 ± 4,247	6,147 ± 2,284	0.72	26,421 ± 10,123	26,305 ± 10,450	>0.99

Cytokines and markers for systemic organ injury were measured in plasma and/or lung homogenates 24 and 72 h post-infection. Data are means ± SD (cytokines) or median (range) of five to eight mice per group. Actual P values are shown, determined via t test or Mann-Whitney U where appropriate; ND, not detectable or below detection limit; TNF-α, tumor necrosis factor-alpha; MCP-1, monocyte chemo-attractive protein-1; IL, interleukin; IFN-γ, interferon-gamma; AST, aspartate transaminase; ALT, alanine transaminase; LDH, lactate dehydrogenase; CXCL1, chemokine (C-X-C motif) ligand 1.

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