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Table 1 Characteristics of the experiment regarding contamination model and type of instillation fluid

From: A simple and easy in vitro model to test the efficacy of IV lines' needleless connectors against contamination

Contamination degree Instillation fluid Colonizing microorganisms MTP (IQR), h Manipulator hand flora
Handsa Saline S. epidermidis, S. warnerii 76.25 (65.92 to 125.01) S. epidermidis, S. warnerii, CoNS, SV, P. acnes, Micrococcus sp.
Propofol Micrococcus sp. 74.91 (51.45 to 99.00) S. epidermidis, S. warnerii, CoNS, SV, P. acnes, Micrococcus sp.
0.5 MF SAb Saline MSSA (ATCC 29213) 104.92 (45.97 to 239.94) NA
Propofol MSSA (ATCC 29213) 147.89 (66.04 to 236.58) NA
0.05 MF SAc Saline MSSA (ATCC 29213) 240.20 (154.82 to 360.00) NA
Propofol MSSA (ATCC 29213) 66.11 (58.01 to 69.11) NA
Controld Saline NA NA NA
Propofol NA NA NA
  1. aManipulation of the connector without gloves. bManipulation of the connector with gloves impregnated with a 0.5 McFarland solution of Staphylococcus aureus ATCC 29213. cManipulation of the connector with gloves impregnated with a 0.05 McFarland solution of Staphylococcus aureus ATCC 29213. dStandard sterile manipulation with clean gloves and with disinfection of connectors using alcohol. MTP, median time to positivity; IQR, interquartile range; SA, Staphylococcus aureus; MF, McFarland; CoNS, coagulase-negative staphylococci.