Table 2 Primer sequences for qPCR analysis
From: Critical illness-induced bone loss is related to deficient autophagy and histone hypomethylation
Gene name | Forward (5’-3’) | Reverse (3’-5’) |
|---|---|---|
Human | ||
TRAF6 | GCAAGTATGAATGCCCCATC | TGGACATTTGTGACCTGCAT |
CD16B | CATTCCAAAAGCCACACTCA | CCCAGGTGGAGAGAATGATG |
NFATc1 | GCATCACAGGGAAGACCGTGTC | GAAGTTCAATGTCGGAGTTTCTGAG |
SQSTM1 | CGTCTGCCCAGACTACGACT | GTGTCCGTGTTTCACCTTCC |
ATG3 | CCACGATTATGGTTGTTTGG | GTGGCAGATGAGGGTGATTT |
ATG7 | ACCTTGGGTTGCAATGTAGC | CTTACCACCCCCTAGGCAAT |
Rabbit | ||
RUNX2 | CTTCACAAATCCTCCCCAAG | ATGCGCCCTAAATCACTGAG |
OSX | ATGGCGTCCTCTCTGCTTG | GCTTCTCGTGCCTGCTTT |
COLA1 | Life Technologies (Paisley, UK): Reference number. OC 03 396116_A 1 | |
OPG | GTGCACTTGGTTTGCTGCTA | CCTGAAGAATGCCTCCTCAC |
RANKL | AAAATGAAGATTTGCAAGACG | TTTCTCGGCTCTCAGGTGTT |
DAP12 | CTGGTGTTGACCCTGCTCAT | TGACTCGGTCTCAGTGATGC |
CD16B | GACCTCCACATTCCAGAAGC | GAGAACGGAGGACGAGATGA |