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Fig. 2 | Intensive Care Medicine Experimental

Fig. 2

From: The role of dexamethasone in scorpion venom-induced deregulation of sodium and water transport in rat lungs

Fig. 2

Alpha-ENaC and NKCC1 lung expression. Semiquantitative immunoblotting of membrane fractions prepared from lung tissue samples from rats in the groups venom (Vn, n = 8), dexamethasone + venom (Dx + Vn, n = 6), and control (Cont, n = 4). a Densitometric analysis revealing significantly lower α-ENaC expression in the Vn group and no statistical difference between the Dx + Vn and Cont groups. *P < 0.05 compared with Cont and Dx + Vn. b Immunoblots reacted with anti-α-ENaC, revealing a 90-kDa band. c Densitometric analysis showing that NKCC1 expression was significantly higher in the Vn group and significantly lower in the Dx + Vn group. *P < 0.01 vs. Cont; **P < 0.001 vs. Dx + Vn; ***P < 0.05 vs. Cont. d Immunoblots reacted with anti-NKCC1, revealing a 170-kDa band and anti-Actin, 43-kDa

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