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Intensive Care Medicine Experimental
Fig. 4 | Intensive Care Medicine Experimental

Fig. 4

From: Heparins attenuated histone-mediated cytotoxicity in vitro and improved the survival in a rat model of histone-induced organ dysfunction

Fig. 4

In vitro effects of histone H3 on endothelial cell. Merged phase-contrast and immunofluorescence images of the rat aortic vascular endothelial cells. Confluent monolayers of cells (left) were incubated with calf thymus histone (25 [middle] and 50 [right] μg/mL). The endothelial cells shrank and became sparse at 6 h after treatment with 50 μg/mL of histone H3. The endothelial cells death was detected microscopically by 7-amino actinomycin D (7-AAD, red) (objective lens ×40)

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