Fig. 5

Carbohydrate metabolites in Drosophila melanogaster after septic injury with Staphylococcus aureus. a We measured glucose and glycogen concentrations at 18, 48, and 168 h in triplicate. We determined glycogen by measuring glucose level after digesting glycogen with amyloglucosidase into free glucose. Level of glucose significantly increased by 168 h in sham flies compared to 18 h. By 168 h, glucose content was significantly lower in infected with antibiotics flies compared to the sham group. Glycogen, triglyceride, protein, and pyruvate levels at 168 h were similar between the two groups. Lactate and LDH activity were significantly elevated by 168 h after surviving sepsis (infected with antibiotics group) (*p < 0.05). Interestingly, the ATP content was significantly lower in infected with antibiotics flies by 48 h compared to sham; however, this difference was lost by 168 h. b There was no difference in the expression of Glut-1 and Glut-3 by 168 h between sham and infected with antibiotics flies