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Intensive Care Medicine Experimental
Fig. 1 | Intensive Care Medicine Experimental

Fig. 1

From: Modulation of monocytes in septic patients: preserved phagocytic activity, increased ROS and NO generation, and decreased production of inflammatory cytokines

Fig. 1

Analysis charts to detect the production of ROS and NO in monocytes and subpopulations. Individual cells (singlets) were selected, and monocytes were characterized by light scatter and side positivity for CD14 (a). Another side versus forward light scatter plot was used to exclude smaller cells (b). CD163+ and CD163− subsets were established based on isotype control (c). ROS generation was measured by GMFI of DCFH represented in histogram graphics for the CD14+ monocytes and the monocyte subsets CD14+ CD163+ and CD14+ CD163−. The graphics are representative of an experiment for ROS detection in healthy volunteers and septic patients in unstimulated cells and after stimulation with P. aeruginosa

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