Fig. 2

Diaphragm muscle cross-sectional area (CSA) in diaphragm skeletal muscle myofibers. In each of the sections, fibers reacting with the antibody appear red, whereas fibers not reacting with the antibody appear black. All fibers are outlined by an antibody reactive to laminin (ab11575, Abcam) which appear green. Scale bars represent 50 μm. CON control animals, CMV controlled mechanical ventilation, CMVC CMV treated with calpeptin. Each bar represents 200–240 fibers. CMV rats received corresponding volumes of vehicle. ***p < 0.001, #p < 0.05