Fig. 4

Granulocyte and monocyte adsorptive apheresis (GMA) alleviated inflammation in the lungs. a The levels of neutrophils and macrophages in the lung were analyzed by flow cytometry at 48 h after CLP (n = 7–9 per group). b Gating strategy for identifying neutrophils and macrophages in the lung. Granulocyte marker [HIS48] and CD45 double-positive cells were defined as neutrophils. Macrophage marker [HIS36] and CD45 double-positive cells were defined as macrophages of rats. c Total cell counts in BALF at 48 h after CLP (n = 7–9 per group). d Total protein in BALF at 48 h after CLP (n = 7–9 per group). e Inflammatory-associated cytokines in the BALF were measured by ELISA at 48 h after CLP (n = 7–9 per group). *p < 0.05, **p < 0.01. Data are expressed as mean ± SEM. CLP cecal ligation and puncture, BALF broncho alveolar lavage fluid, ELISA enzyme-linked immunosorbent assay