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Intensive Care Medicine Experimental
Fig. 3 | Intensive Care Medicine Experimental

Fig. 3

From: Regulation of soluble CD127 protein release and corresponding transcripts expression in T lymphocytes from septic shock patients

Fig. 3

sCD127 release and transcripts expression upon ex vivo stimulation of healthy volunteers’ purified T cells. a sCD127 protein release and b IL7R3 and IL7R7 mRNA transcripts expressions were measured in purified T cells from healthy volunteers (HV) stimulated ex vivo after the indicated time of incubation with IL-7 (n = 5, 10 ng/mL), anti-CD3/CD28 antibodies coated beads (n = 5, αCD3/28, 1:2 bead to cells ratio), or not stimulated (n = 10, NS). For each HV and each condition of stimulation, samples were proceeded in biological triplicates (culture wells) and technical duplicates (for ELISA only), summarized by the mean of the values for each individual. Data are presented as means and standard error of the mean. Mann-Whitney tests were used to compare values between non-stimulated and stimulated purified T cells from HV. *p < 0.05, **p < 0.01, ***p < 0.001. See Additional file 1 for details

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