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Intensive Care Medicine Experimental
Fig. 3 | Intensive Care Medicine Experimental

Fig. 3

From: Cytokine pre-activation of cryopreserved xenogeneic-free human mesenchymal stromal cells enhances resolution and repair following ventilator-induced lung injury potentially via a KGF-dependent mechanism

Fig. 3

Pre-activated, cryopreserved, XF-hMSCs enhance resolution of alveolar neutrophil infiltration and modulate BAL inflammatory cytokines. All hMSC treatment groups significantly reduced lung neutrophil infiltration (a). All treatment groups significantly decreased BAL concentrations of CINC-1 (b) and IL-6 (c). For IL-10, KGF and PGE2, fresh MSC treatment did not restore release (d–f). However, pre-activated fresh MSCs showed significant recovery of IL-10 (d), KGF (e) and PGE2 (f) concentrations. Neither frozen nor activated frozen MSC delivery recovered IL-10, KGF or PGE2 release (d–f). *, ** and ***P < 0.05, 0.01 and 0.001, respectively, versus PBS control; $ and $$P < 0.05 and 0.01, respectively, versus naive fresh group. Sham, n = 3–6; PBS control, n = 6–8; fresh, n = 6–8; fresh pre-activated, n = 5–8; cryopreserved, n = 5–6; cryopreserved pre-activated, n = 6–8

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